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. 2016 Oct 31;18(1):39–47. doi: 10.15252/embr.201642412

Figure 1. CD1d expression on ILCs.

Figure 1

  1. Flow cytometry for CD1d expression (top) and CD1d mean fluorescence intensity (MFI; bottom) on CD45− cells, ILCs (Lin−CD45+CD127+), DCs (CD11c+) and B cells (B220+) of the depicted tissues from WT mice (n = 4).
  2. Flow cytometry profiles showing gating strategy (dot plots), CD1d expression (histograms) and CD1d MFI (bars) in T‐bet+ (empty profile), GATA‐3+ (dark grey), RORγt+ (light grey) and T‐bet+RORγt+ (dotted line) ILCs in mLN, spleen, PP and SI‐LP (n = 5). Numbers indicate percentage of cells in the depicted gates.
  3. Quantitative RT–PCR analysis of mRNA encoding CD1d in freshly isolated ILC3s (from mLN of CD1d‐deficient mice and mLN and spleen of WT mice), B cells and DCs (n = 3). Results are normalized to those of GAPDH; RE, relative expression. **P < 0.01 (two‐tailed unpaired t‐test).
  4. Flow cytometry profiles showing NKp46 and RORγt expression in Lin−CD45+ cells; CD1d and CCR6 expression in NCR− ILC3s; and CD1d and CD4 expression in NCR−CCR6+ ILC3s from mLN and SI‐LP (n = 4). Numbers indicate percentage of cells in the depicted gates.
  5. CD1d expression (histograms) and CD1d MFI in NCR+ (empty profile), NCR−CCR6− (dark grey) and NCR−CCR6+ (light grey) ILC3s from mLN and SI‐LP (n = 4).
Data information: Graphs represent mean ± SEM. Data are from three (A, C–E) or five (B) independent experiments.