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. 2016 Oct 31;18(1):39–47. doi: 10.15252/embr.201642412

Figure EV1. CD1d expression on ILCs.

Figure EV1

  1. Flow cytometry profiles showing gating strategy and CD1d expression (histogram) in Lin−CD45+CD127+ cells in the depicted tissues from WT (grey filled, n = 4) and CD1d‐deficient (empty profile, n = 4) mice.
  2. Flow cytometry profiles showing gating strategy and CD1d expression (histograms) in T‐bet+ (empty profile), T‐bet+RORγt+ (dotted line), GATA‐3+ (dark grey) and RORγt+ (light grey) ILCs in colonic lamina propria (n = 3). Numbers indicate percentage of cells in the depicted gates.
  3. Flow cytometry profiles showing expression of CD1d and T‐bet in Lin−CD45+CD127+RORγt+ cells from mLN, spleen and SI‐LP as indicated (n = 4); numbers indicate percentage of cells. Right panel, percentage of CD1dhi ILC3s in the depicted tissues. ***P < 0.001 two‐tailed unpaired t‐test.
  4. Relative expression (RE) of lymphotoxin‐alpha (LTA) mRNA (qPCR, normalized to GAPDH) in sort‐purified CD1dhiCCR6hi (light grey) and CD1dlowCCR6low (dark grey) ILC3s isolated from mLN or spleen; **P < 0.01, two‐tailed unpaired t‐test.
Data information: Graphs represent mean ± SEM. Data are from three independent experiments.