Figure 5. AhR deficiency impairs BCR‐dependent B‐cell proliferation in vivo .

• A, B
  Flow cytometry analysis of distribution of CD45.1⁺ and CD45.2⁺ cells in indicated cell subsets purified from bone marrow (A) and lymph node (B) of sublethally irradiated Rag1 ^−/− mice 8 weeks after reconstitution with equal numbers of bone marrow cells from Ahr ^+/+ (CD45.1⁺) and Ahr ^−/− (CD45.2⁺) mice. Dashed lines indicate 50% level. Representative data of n = 2 independent experiments; two‐tailed paired t‐test.
• C
  Host CD45.1 mice were co‐transferred with a 1:1 mixture of HEL‐specific Ahr ^+/+ CD45.1⁺CD45.2⁺ splenocytes isolated from SW _HEL Ahr ^+/+ mice and HEL‐specific Ahr ^−/− CD45.2⁺ splenocytes isolated from SW _HEL Ahr ^−/− mice in the presence of SRBC‐HEL or SRBC‐mock. Readout at d7 post‐challenge was distribution of Ahr ^+/+ CD45.1⁺CD45.2⁺ vs. Ahr ^−/− CD45.2⁺ cells.
• D
  Flow cytometry analysis of distribution of CD45.1⁺CD45.2⁺ and CD45.2⁺ cells harvested from host mice challenged as indicated above the dot plots. Representative data of n = 3 independent experiments.
• E
  Flow cytometry analysis of distribution of Ahr ^+/+ CD45.1⁺CD45.2⁺ (black) and Ahr ^−/− CD45.2⁺ (white) cells harvested from host mice. Indicated distribution is quantified relative to transferred cells. X symbols indicate SRBC‐mock‐treated control. Dashed line indicates 50% threshold. Representative data of n = 3 independent experiments; two‐tailed paired t‐test.
• F, G
  Flow cytometry analysis of distribution (F) and cell numbers (G) of IgG1⁺ and IgG1⁻ HEL3x‐binding Ahr ^+/+ CD45.1⁺CD45.2⁺ (black) and Ahr ^−/− CD45.2⁺ (white) cells harvested from host mice. Representative data of n = 2 independent experiments; two‐tailed paired t‐test.