Skip to main content
. 2016 Dec 15;18(1):72–86. doi: 10.15252/embr.201642681

Figure EV2. Osmotic stress induces YAP and TAZ nuclear translocation in serum‐free conditions.

Figure EV2

  1. NaCl induces YAP nuclear translocation under serum‐starved conditions. HEK293A cells were serum starved for 1 h followed by 0.1 M NaCl treatment for 1 h. YAP/TAZ subcellular localization was determined by immunofluorescence staining (red). Scale bars: 20 μm.
  2. Quantification of Fig 2B. YAP knockout (KO) HEK293A cells were serum starved for 1 h followed by 0.2 M sorbitol treatment for 1 h. Quantification of more nuclear (N > C) or more cytosolic (N < C) YAP signal was determined with randomly chosen fields.
  3. YAP expression is completely abolished in YAP knockout (KO) cells. Western blot shows that YAP is absent and TAZ is still present in the YAP KO HEK293A cells. Vinculin serves as a loading control.