Figure 5. YAP Ser128 phosphorylation affects YAP subcellular localization.

1. NLK does not induce nuclear translocation of the S128A mutant YAP. HEK293A cells were co‐transfected with Myc‐NLK and Flag‐YAP WT or S128A mutant. Cells were serum starved for 6 h, and YAP localization and NLK expression were determined by Flag (green) and Myc (red) antibodies, respectively. Scale bars: 20 μm.
2. YAP Ser128 phosphorylation is required for osmotic stress‐induced YAP nuclear localization. HEK293A YAP KO cells reconstituted with Flag‐YAP WT, YAP S128D, or YAP S128A mutant were treated with 0.2 M sorbitol for 1 h. YAP subcellular localization was determined by Flag immunofluorescence staining (green). Scale bars: 20 μm.