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. 2016 Dec 19;18(1):150–168. doi: 10.15252/embr.201642360

Figure 2. GSCs are hypersensitive to PIs compared with their DFCs.

Figure 2

  • A–H
    Cell viability assays were performed as described in the Materials and Methods after cells were treated with temozolomide (A), cisplatin (B), adriamycin (C), taxol (D), TRAIL (E), bafilomycin A1 (F), thapsigargin (G), and MG132 (H). Means ± SD from at least three independent experiments are shown as relative indexes after normalization to those of individual control cells treated with DMSO.
  • I
    XO8 GSCs and DFCs were treated with 50 nM MG132 for 24 h. Cell viability was measured using trypan blue exclusion assay. Error bars represent the mean ± SD from three independent experiments. For statistical analysis, Student's t‐test (two‐sided, paired) was used (*P < 0.05).
  • J
    XO8 GSCs were treated with MG132 were cultured in 0.3% soft agar as described in the Materials and Methods. The colonies bigger than 100 μm in diameter in four randomly chosen fields were subjected to blind counting. Bars, means + SD from three independent experiments.