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. 2016 Dec 19;18(1):150–168. doi: 10.15252/embr.201642360

Figure 6. The expressions of ER stress‐associated proapoptotic genes are selectively induced by MG132 in GSCs.

Figure 6

  • A, B
    XO8 GSCs and XO8 DFCs were treated with 50 nM MG132 for indicated hours. The expression of genes was assessed by real‐time PCR using total RNA extracted from MG132‐treated cells. Mean + SD values from the three independent experiments are shown after normalization to those of control cells treated with DMSO.
  • C
    XO8 GSCs and XO8 DFCs treated with 50 nM MG132 as described in (A) were subjected to immunoblotting.
  • D
    XO8 GSCs were treated with MG132, salinosporamide A, epoxomicin, or bortezomib for 24 h. Cell lysates were subjected to immunoblot analysis with anti‐PARP‐1, anti‐CHOP, anti‐ATF3, anti‐ATF4, and anti‐BiP antibodies.
  • E
    XO8 cells were treated with 50 nM MG132 in the absence or presence of 5 μM actinomycin D (ActD) for 6 h. The level of ATF3 mRNA was measured using semi‐quantitative real‐time PCR in comparison with β‐actin. Mean + SD values from three independent experiments are shown after normalization to those of control cells treated with DMSO.

Source data are available online for this figure.