Figure EV1. Myeloid cells are the main source of IL‐1β upon MOG/CFA/PTx immunization.

• A–C
  Analysis of IL‐1β expression by cells isolated from the dLN and stimulated with GM‐CSF (A), LPS (B), and PMA/ionomycin (C). Data are representative FACS plots gated on VD⁻ cells with mean frequencies per group.

Data information: Cells (A–C) were isolated at day 7 after immunization and stimulated in the presence of monensin with indicated stimuli for 4 h. Data consist of n = 4 WT PBS‐, n = 3 IL‐1R1^−/− PBS‐, n = 4 WT PTx‐, n = 3 IL‐1R1^−/− PTx MOG/CFA‐immunized mice. Experiments were performed twice with similar results.