Figure EV1. TRIM16 mediates IL‐1β secretion.

• A
  Knockdown efficacies as determined by immunoblots.
• B
  LDH release data for samples in Fig 1A.
• C, D
  Levels of (C) IL‐1β and (D) LDH release were determined in supernatants from THP‐1 cells subjected to knockdowns as indicated, treated with 100 ng/ml LPS overnight, then starved in EBSS for 3 h.
• E
  LDH release results for samples in Fig 1C.
• F
  Co‐IP analysis of interactions between flag‐TRIM16 with myc‐pro‐IL‐1β in HEK293T cells.
• G
  Immunoblot analyses of knockdown efficacies in THP‐1 cells.
• H
  LDH release data for samples in Fig 1E.
• I
  Immunoblot analyses of the levels of mIL‐1β in supernatants from THP‐1 cells that were subjected to knockdown as indicated and were sequentially treated with LPS and LLOMe.
• J
  LDH release data from samples in Fig 1F.
• K, L
  Levels of (K) IL‐1β and (L) LDH release was determined in supernatants from THP‐1 cells subjected to knockdown as indicated, treated with 100 ng/ml LPS overnight, then starved in EBSS for 3 h.
• M
  Immunoblot analyses of knockdown efficacies in primary human MDM cells.
• N
  LDH release results for samples in Fig 1G.
• O
  Intracellular localization analysis of TRIM16, pro‐IL‐1β, and LAMP2 by confocal microscopy. Cells, THP‐1. Line tracings correspond to arrows. Scale bars, 5 μm.

Data information: Means ± SEM; n ≥ 5, except for immunoblot quantifications and TRIM screen where n ≥ 3. *P < 0.05, ^† P ≥ 0.05 (t‐test for N; ANOVA for B, C, D, H, J, K, L).