Figure EV2. Galectin‐8 participates in IL‐1β secretion and interacts with TRIM16.

• A
  Immunoblot analyses of knockdown efficacies in THP‐1 cells.
• B, C
  LDH release data for samples in (B) Fig 2A and (B) Fig 2B.
• D
  Immunoblot analyses of knockdown efficacies in primary human MDMs.
• E
  LDH release data for samples in Fig 2C.
• F
  Confocal microscopy of HeLa cells treated with LLOMe and stained for galectin‐8 and HSP90. Arrowheads in enlarged insets indicate colocalization. Scale bars, 5 μm.
• G
  Galectin‐8 domains and deletion constructs used.
• H
  In vitro translated and radiolabeled [³⁵S] myc‐HA‐TRIM16 was incubated with full‐length‐ and deletions of GST‐galectin‐8 in the presence of flag‐ULK1 and cold ATP, and GST pull downs were performed and [³⁵S] radiolabeled Myc‐HA‐TRIM16 detected by SDS‐PAGE and autoradiography. Amounts of GST fusion proteins are shown in Coomassie brilliant blue (CBB)‐stained gels.

Data information: Means ± SEM; n ≥ 5, except for immunoblot quantifications where n ≥ 3. ^† P ≥ 0.05 (ANOVA for B, C, E).