-
A, B
IL‐1β levels in supernatants of THP‐1 cells subjected to knockdowns, treated with LPS, and then (A) with LLOMe or (B) starved in EBSS.
-
C
IL‐1β levels in supernatants of primary human macrophages (MDM) subjected to knockdowns and sequentially treated with LPS and LLOMe.
-
D
Immunoblot analyses of p62 levels in THP‐1 cells subjected to knockdowns as indicated and starved for 3 h.
-
E
mRFP‐GFP overlapping areas of tandem HeLa cells reconstituted with flag‐pro‐IL‐1β and myc‐caspase‐1 and starved for 3 h. Mask overlay, iDev software‐defined objects [primary objects, cell outlines; internal secondary objects, GFP (top), mRFP (middle), or their overlapping area (bottom)]. Scale bars, 10 μm.
-
F
IL‐1β levels in supernatants of starved tandem HeLa cells reconstituted with flag‐pro‐IL‐1β and myc‐caspase‐1 as in (E).
Data information: Means ± SEM;
n ≥ 5, except for immunoblot quantifications where
n ≥ 3. *
P <
0.05,
†
P ≥
0.05 (
t‐test for C, E, F; ANOVA for A, B).