Skip to main content
. 2016 Dec 8;36(1):42–60. doi: 10.15252/embj.201695081

Figure 4. R‐SNARE Sec22b participates in IL‐1β secretion.

Figure 4

  • A, B
    IL‐1β levels in supernatants of THP‐1 cells subjected to knockdowns, treated with LPS, and then (A) with LLOMe or (B) starved in EBSS.
  • C
    IL‐1β levels in supernatants of primary human macrophages (MDM) subjected to knockdowns and sequentially treated with LPS and LLOMe.
  • D
    Immunoblot analyses of p62 levels in THP‐1 cells subjected to knockdowns as indicated and starved for 3 h.
  • E
    mRFP‐GFP overlapping areas of tandem HeLa cells reconstituted with flag‐pro‐IL‐1β and myc‐caspase‐1 and starved for 3 h. Mask overlay, iDev software‐defined objects [primary objects, cell outlines; internal secondary objects, GFP (top), mRFP (middle), or their overlapping area (bottom)]. Scale bars, 10 μm.
  • F
    IL‐1β levels in supernatants of starved tandem HeLa cells reconstituted with flag‐pro‐IL‐1β and myc‐caspase‐1 as in (E).
Data information: Means ± SEM; n ≥ 5, except for immunoblot quantifications where n ≥ 3. *P < 0.05, † P ≥ 0.05 (t‐test for C, E, F; ANOVA for A, B).