-
A
Immunoblot analysis of the levels of FTH1 in supernatants from THP‐1 cells that were subjected to knockdown as indicated and were sequentially treated with LPS and LLOMe.
-
B
Ferritin levels in supernatants of primary human MDM macrophages subjected to knockdowns as indicated and treated sequentially with LPS and LLOMe.
-
C
Ferritin levels in supernatants of THP‐1 cells subjected to knockdowns as indicated and infected with M. tuberculosis Erdman as indicated.
-
D, E
(D) Ferritin levels and (E) LDH release in supernatants of primary human MDM cells subjected to knockdowns as indicated and infected with M. tuberculosis as indicated.
-
F, G
(F) Ferritin levels and (G) LDH release in supernatants of primary human MDM cells infected with M. tuberculosis as indicated.
-
H
LDH release data from Fig
6E.
-
I, J
Immunoblot analyses of knockdown efficacies in THP‐1 cells.
-
K, L
Levels of (K) IL‐1β and (L) LDH release determined in supernatants from THP‐1 cells subjected to knockdowns as indicated and were sequentially treated with LPS and LLOMe.
-
M
Co‐IP analysis of interaction between FTH1 with GFP‐TRIM16, GFP‐galectin‐8, and GFP‐Sec22b.
Data information: Means ± SEM;
n ≥ 5, except for immunoblot quantifications where
n ≥ 3. *
P <
0.05,
†
P ≥
0.05 (vs. LLOMe‐treated or Mtb‐infected scr control or indicated) (
t‐test for F–H, K, L; ANOVA for B–E).