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. 2016 Dec 8;36(1):42–60. doi: 10.15252/embj.201695081

Figure EV6. Secretory autophagy machinery plays a role in unconventional secretion of ferritin.

Figure EV6

  • A
    Immunoblot analysis of the levels of FTH1 in supernatants from THP‐1 cells that were subjected to knockdown as indicated and were sequentially treated with LPS and LLOMe.
  • B
    Ferritin levels in supernatants of primary human MDM macrophages subjected to knockdowns as indicated and treated sequentially with LPS and LLOMe.
  • C
    Ferritin levels in supernatants of THP‐1 cells subjected to knockdowns as indicated and infected with M. tuberculosis Erdman as indicated.
  • D, E
    (D) Ferritin levels and (E) LDH release in supernatants of primary human MDM cells subjected to knockdowns as indicated and infected with M. tuberculosis as indicated.
  • F, G
    (F) Ferritin levels and (G) LDH release in supernatants of primary human MDM cells infected with M. tuberculosis as indicated.
  • H
    LDH release data from Fig 6E.
  • I, J
    Immunoblot analyses of knockdown efficacies in THP‐1 cells.
  • K, L
    Levels of (K) IL‐1β and (L) LDH release determined in supernatants from THP‐1 cells subjected to knockdowns as indicated and were sequentially treated with LPS and LLOMe.
  • M
    Co‐IP analysis of interaction between FTH1 with GFP‐TRIM16, GFP‐galectin‐8, and GFP‐Sec22b.
Data information: Means ± SEM; n ≥ 5, except for immunoblot quantifications where n ≥ 3. *P < 0.05, † P ≥ 0.05 (vs. LLOMe‐treated or Mtb‐infected scr control or indicated) (t‐test for F–H, K, L; ANOVA for B–E).