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. 2016 Dec 15;18(1):169–183. doi: 10.15252/embr.201642586

Figure EV1. Trif‐mutant neurons lose the response to poly(I:C) stimulation.

Figure EV1

At 5 DIV, Trif‐mutant neurons were treated with poly(I:C) for 6 h, RNAs were extracted, and the expression levels of Tnfa, Ifnb, and Il1b were examined by using quantitative RT‐PCR. The scales of y‐axes are identical to those in Fig 4A for the responses of wild‐type neurons to poly(I:C) and saline control. For Ifnb, the expression levels in response to poly(I:C) decreased dramatically from 100‐fold to 4‐fold relative to Hprt transcripts when comparing wild‐type and Trif‐mutant neurons; although, compared with saline control, poly(I:C) still increased Ifnb expression in Trif‐mutant neurons. Data were analyzed by unpaired t‐test. N = 6. Mean values ± SEM are shown. **P < 0.001.