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A
Representative image of a mouse spheroid stained for β‐catenin (green) and F‐actin (red). Nuclei are visualized with bisbenzimide (blue). A similarly stained section of a mouse villus is shown for comparison. Arrowheads indicate the apical cell membrane. Scale bars, 50 μm.
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B, C
Mouse jejunal spheroids were cultured as indicated. (B) Quantification of the average expression ± s.e.m. of Fabp1, Ace2, and Maoa mRNAs (n = 3 independent experiments). *P < 0.05, **P < 0.01 by one‐way ANOVA and Tukey's post‐test. (C) Representative images of spheroids stained for Ace2 (green) and β‐catenin (red). Nuclei are visualized with bisbenzimide (blue). Scale bars, 20 μm.
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D, E
Graphs showing the top five most significant pathways (D) and gene ontology cellular component terms (E) associated with Cluster 3.
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F, G
Representative TEM images of EP4i‐treated spheroids. (F) The basal plasma membranes are outlined in orange solid lines, lateral plasma membranes are indicated with orange arrowheads, and nuclei are outlined with wide yellow dashed lines. Inset shows a magnified view of the apical cell surface. (G) Higher power image of the cytoplasm. Single mitochondria are outlined with a narrow blue dashed line. Scale bars, 1 μm.
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H
Graph of spheroid oxygen consumption rate (OCR) to extracellular acidification rate (ECAR) ratio expressed as mean ± s.e.m. (n = 3 independent experiments). **P < 0.01 by one‐way ANOVA and Tukey's post‐test.
