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. 2016 Dec 5;18(1):138–149. doi: 10.15252/embr.201643206

Figure 2. The MltE LTG is required for T6SS function.

Figure 2

Hcp release assay. FLAG‐tagged Hcp (HcpFL) release was assessed by separating cells (C) and cell‐free culture supernatant (S) fractions from 109 cells of the indicated strains. Proteins were separated by 12.5% acrylamide SDS–PAGE and TolB and HcpFL were immunodetected using anti‐TolB (upper panel) and anti‐FLAG (lower panel) antibodies. Molecular weight markers (in kDa) are indicated on the left. The experiment was performed in triplicate and a representative result is shown.