TABLE I.
Gene | ACC. No. | siRNA, primer or probe | Sequence |
---|---|---|---|
Sox9 | AF278703 | siRNA S1 | 5′-GCGTCAACGGCTCGAGCAA-3′ |
siRNA S2 | 5′-TGACCGACGAGCAGGAGAA-3′ | ||
siRNA S3 | 5′-GGAAGTCGGTGAAGAACGG-3′ | ||
siRNA S4 | 5′-GACTGCTGAACGAGAGCGA-3′ | ||
Not applicable | Not applicable | Non-targeting siRNA | 5′-TGGTTTACATGTCGACTAA-3′ |
Sox9 | AF278703 | Forward primer | 5′-AATCTCCTGGACCCCTTCATG-3′ |
Reverse primer | 5′-GGCGGACAGGCCCTTCT-3′ | ||
Probe | 5′-AGATGACCGACGAGCAG-3′ | ||
Col1α1 | NM_001034039 | Forward primer | 5′-AAAAGAGGCACGTCTGGTAC-3′ |
Reverse primer | 5′-CGGATTCCAGTTCGAGTATGGCGG-3′ | ||
Probe | 5′-GTGGTAGGTGATGTTCTGGG-3′ |