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. Author manuscript; available in PMC: 2017 Nov 17.
Published in final edited form as: Mol Cell. 2016 Nov 17;64(4):659–672. doi: 10.1016/j.molcel.2016.10.019

Figure 4. EPOP recruits Elongin BC to chromatin.

Figure 4

A,B) Western blotting of proteins and histone modifications in control cells, Epop KO cells, and KO cells re-expressing either wildtype (WT) or L40A mutated EPOP. Actin or H3 is shown as loading control. C) Overlap of Elongin B ChIP-Seq peaks (brown) with RNA Polymerase II (blue) and EPOP (red) or SUZ12 (orange) and EZH2 (purple). D) Heatmaps of Elongin B ChIP-Seq in control, Epop KO cells and the subtraction (KO – control). The right panel shows the promoters profiles of Elongin B in control and KO cells (two replicates combined). The P values were calculated by ANOVA. See also Figure S5B C) Upper part: Genome browser view at Tet1 and Esrrb gene locus of ChIP-Seq data for EPOP, H3K4me3 and Elongin B in control and Epop KO cells. Lower part: ChIP-qPCR for Elongin B, EPOP and H3K4me3 at the Esrrb and Tet1 gene locus, in the four cell lines described in A). The primer positions are shown above. The values represent mean ± SD of two biological replicates. See also Figure S5.