Fig. 2.

Restoring of TAG synthesis in H1266 yeast mutant by N. oceanica DGTT1-6 and PDAT1 over-expression. a Quantification of TAG levels extracted from transformed yeast shown as fatty acids esterified to TAG (TAG FA) over total fatty acids (FA total). DGTT proteins were present in each case but varied as determined by immunoblotting (Additional file 7: Figure S6). Tripentadecanoin (tri15:0) TAG was added as internal standard (n = 3; average ± SD). H1266 + EV indicates the empty vector control. b Changes in the fatty acid profile between wild-type and transformed yeast. c Lipid droplets formation analyzed by Nile Red staining (green) in H1266 cells, H1266 strain transformed with empty vector and H1266 cells expressing NoDGTT5 gene. Bars 5 μm. d Competition assay with unlabeled substrate CoAs. The composition of synthesized TAG was analyzed by UHPLC-nano ESI-MS/MS and is given as relative content of each used CoA in total pool of newly synthesized TAG. For all experiments values represent mean ± SD (n = 3)