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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Comp Biochem Physiol C Toxicol Pharmacol. 2016 Sep 21;191:194–202. doi: 10.1016/j.cbpc.2016.09.007

Fig. 2.

Fig. 2

Metal insufficiency in C. elegans causes fertility defects. (A) Experimental setup: worms were grown on either control plates or on plates containing 10 μM TPEN to sequester available transition metal ions. (B) Total number of progeny produced by either untreated or 10 μM TPEN treated animals. Increased TPEN concentration incrementally reduces brood size from day 1 to day 5 (day 1 **p = 0.0024, day 2 **p = 0.0033, ****p < 0.0001, day 3 *p = 0.0279, **p = 0.0023, ****p < 0.0001, day 4 *p < 0.0153). (C) The concentration of TPEN in the growth media was varied to observe dose response effects. Fertility was measured for the first 5 days after L4 molt.