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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Comp Biochem Physiol C Toxicol Pharmacol. 2016 Sep 21;191:194–202. doi: 10.1016/j.cbpc.2016.09.007

Fig. 6.

Fig. 6

Mild zinc insufficiency has a small impact on C. elegans male sperm counts. (A) Distribution of sperm counts in gonad arms of either control males or males treated with 10 μM TPEN (zinc insufficient = ZI). N represents the number of gonad arms measured. Bars and error bars represent the mean and standard deviation of the distribution, respectively. The difference in the distributions was found to be statistically insignificant by Student's t-test. (B) Representative images of fixed C. elegans males stained with Hoechst, demarking the sperm. Control or zinc insufficient males (10 μM TPEN) do not have visually different numbers of sperm. Scale bar is 20 μm.