Fig. 2.

Immunoreactivity of plasma oxidized AGT (oxi-AGT) and red-AGT to an anti-ANG I antibody. The immunoreactivity of oxi-AGT and red-AGT to an anti-AGT antibody and an anti-ANG I antibody was tested. Mouse plasma was used in the experiments. One microliter of the plasma sample was incubated at 70°C with lithium dodecyl sulfate (LDS) and 50 mM DTT, a reducing reagent, to denature plasma AGT and dissociate disulfide bonds in AGT. Another sample was prepared without DTT. After gel electrophoresis and protein transfer from the gel to a membrane in the absence of DTT, AGT proteins were detected by an anti-AGT antibody (A) and anti-ANG I antibody (B). M, molecular weight markers. Furthermore, dot blot assays were also performed using these antibodies (C and D).