General, renal, and inflammatory parameters in wild-type and hepcidin knockout mice. Included are body weight (A), serum urea nitrogen (B), serum creatinine (C), liver Saa mRNA expression (D), and serum IL-6 (E) for all groups. a: Statistically significant pairwise comparison of low- vs. standard-iron groups within the wild-type control, wild-type chronic kidney diseae (CKD), hepcidin knockout control, and/or hepcidin knockout CKD cohorts; b: statistically significant pairwise comparison of control vs. CKD groups within the wild-type low-iron, wild-type standard-iron, hepcidin knockout low-iron, and/or hepcidin knockout standard-iron cohorts; c: statistically significant pairwise comparison of wild-type vs. hepcidin knockout groups within the CKD low-iron and/or CKD standard-iron cohorts. Given multiple comparison testing, the Benjamini-Hochberg correction was used. Data in A, B, C, and E are presented as medians and interquartile ranges, with box plot whiskers representing the 10th and 90th percentiles. Data in D are presented as means and SE; n = 6–14 mice/group, except where denoted on the graphs.