Fig. 1.

Testosterone increases the depolarization-induced whole cell outward currents in freshly isolated detrusor smooth muscle (DSM) cells. A: representative original recordings illustrating the depolarization-induced whole cell outward currents in the absence (control) and in the presence of 100 nM testosterone. B: current-voltage relationship curve summarizes the stimulatory effects of 100 nM testosterone on the whole cell outward currents (n = 15, N = 6; *P < 0.05). C: representative original recordings illustrating the depolarization-induced whole cell outward currents in the presence of 1 μM paxilline alone and in the presence of both 1 μM paxilline and 100 nM testosterone. D: current-voltage relationship curve summarizes the lack of stimulatory effects of 100 nM testosterone on the whole cell outward currents in the presence of 1 μM paxilline (n = 7, N = 7; P > 0.05). The original recordings represented in A and C are from 2 separate DSM cells.