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. 2017 Jan 3;18:2. doi: 10.1186/s12859-016-1412-z

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — If in the first step of the pruning procedure the flux through reaction 1 is set to zero, reaction 1 is removable and reactions 2 and 3 are non-removable. If in the first step reaction 2 or 3 is set to zero, both of them would be removable and reaction 1 would be non-removable. The resulting subnetwork is then smaller than the first one