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. 2017 Jan 4;10:1. doi: 10.1186/s13041-016-0281-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Transduction of Tat-HSP22 protein into gerbil brain and its protective effects. Gerbils were treated with single injections of Tat-HSP22 (2 mg/kg) or control HSP22 protein and killed. a 7 days after ischemic insults, transduced Tat-HSP22 protein was confirmed using an anti-Histidine, NeuN, DAPI, and Alexa immunostaining. Scale bar = 50 μm. b The changes of cleaved caspase-3 expression levels were confirmed using an anti-cleaved caspase-3 staining after 12 h ischemic insults. Scale bar = 18.8 μm. ^** P < 0.01, significantly different from the vehicle group