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Effect of irisin on AMPK and Akt phosphorylation in human coronary endothelial cells. Endothelial cells were incubated with irisin at the indicated concentrations and times. Phospho‐AMPKα1 (A and B) and phospho‐Akt (C) results were normalized by total AMPKα1 and Akt, respectively (*P<0.05 vs control group; n=4). Akt indicates protein kinase B; AMPK, AMP‐activated protein kinase; DU, density units; p, phospho.
