Figure 1.

Role of liposome quality in antisense transfection efficiency. A, Antisense encapsulation inside the liposomes. Antisense oligodeoxynucleotide labeled with 5′ fluorescein (FITC) was used to ensure that antisense was encapsulated inside the liposomes. Antisense took the shape of liposomes and appeared as green spherical bodies under fluorescent microscopy; their size ranged from 100 to 200 nm. Measurement of the size of the PEG‐cationic liposomes and polydispersity index using dynamic light scattering. B, The size of the freshly formulated liposomes ranged from 100 to 200 nm with a polydispersity index of 0.1.