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. 2016 Nov 10;5(11):e003922. doi: 10.1161/JAHA.116.003922

Figure 6.

Figure 6

miR‐590‐mediated Sp1 is critical for cardiac reprogramming. A, Relative mRNA expression of Sp1 in untreated (mock) porcine cardiac fibroblasts and porcine cardiac fibroblasts transfected with scrambled siRNA or anti‐Sp1 siRNA (1, 5, and 10 μmol/L; n=3). *P<0.05 versus mock (Wilcoxon rank‐sum test). B, Relative mRNA expression of cardiomyocyte marker gene (TNNT2, MYH6) in and porcine cardiac fibroblasts transduced with GMT and/or miR‐590, and with or without si‐Sp1 (n=3). *P<0.05 versus relevant control (Kruskal–Wallis test). C, Relative mRNA expression of fibroblast marker genes (COL1A1 and COL3A1) in, and porcine cardiac fibroblasts transduced with, GMT and/or miR‐590, and with or without si‐Sp1 (n=3). *P<0.05 versus relevant control (Kruskal–Wallis test). D, Left: Representative flow cytometry plots for analyses of cTnT + cells 2 weeks after transduction of porcine cardiac fibroblasts with GMT and si‐Sp1 or miR‐590. Left to right: GFP, GMT, GMT plus miR‐590, and GMT plus si‐Sp1. Right: Summary of flow cytometry analyses. Percentage of cTnT + cells following infection of porcine cardiac fibroblasts (n=3; Wilcoxon rank‐sum test). Data are presented as mean±SEM. *P<0.05 versus relevant control. COL indicates collagen; cTnT, cardiac troponin T; GFP, green fluorescent protein; miR, microRNA; Sp1, specificity protein 1.