FIG 2 .

Role of dynamin in CW internalization. (A) A549 cells (black bars) and HBMEC (white bars) were pretreated with DMSO (Mock) or 80 µM dynasore, incubated with CypHer5E-labeled CW for 2 h at 37°C, and processed and analyzed as described in the legend to Fig. 1C. Data are presented as means plus SD from at least three independent experiments. ****, P < 0.0001 (Student’s t test). (B) WT MLEC (gray bars) and PAFr KO MLEC (checkerboard bars) were treated with CW and dynasore as described above for panel A (left) or dynasore was removed for the inhibitor washout experiment (right). Data are presented as means plus SD from three independent experiments. **, P < 0.01; ****, P < 0.0001 (Student’s t test).