FIG 1 .

Basal levels of ppGpp are increased in VRE strains harboring mutant relA alleles. (A) Multiple relA WT or mutant (isogenic) strains were grown in low-phosphate media, followed by incubation with ³²P, and levels of resting (p)ppGpp were measured by densitometry. (B) Data for ppGpp levels are quantified, and means and standard deviations are shown. Levels were normalized relative to the wild-type strain (100%). *, P = 0.0079 by Mann-Whitney testing. (C) Controls: negative control without any stressor (Cont.), positive-control mupirocin, as well as linezolid.