FIG 4 .

The ClpP activator successfully eradicates biofilm irrespective of the relA mutation. The wild type (A) and an isogenic mutant (B) harboring the relA missense mutation were allowed to form biofilms for 24 h. Following biofilm formation, cells were treated with either DMSO (black lines) or the ClpP activator ADEP-4 at 0.2 µM for 24 h (blue lines). After antibiotic treatment, biofilms were washed, collected by centrifugation, and allowed to outgrow in antibiotic-free medium to ascertain an approximation of bacterial killing. Data represent the mean from four replicates; error bars represent standard deviation.