FIG 5 .

Modulation of miR-203a-3p targets and analysis of miR-203a-3p expression. Effects of a miR-203a-3p mimic (MIM, green) or an LNA inhibitor (light blue) on TP63 (A) and BMI1 (B) levels in HPV16 E6/E7-expressing HFKs. Expression of 18S rRNA was used as an internal control, and values were normalized to a negative control (C) mimic or LNA. TP63 and BMI1 expression was assessed by RT-PCR. (C) Expression of miR-203a-3p in three independently derived HFK populations expressing HPV16 E6, E7, or E6/E7 or a control vector via TaqMan miR assay. Expression of the noncoding snRNA U6 spliceosomal RNA was used as an internal control. Results represent averages of at least three independent experiments.