Figure 1.

The GETPrime 2.0 search interface and tabular display. The figure shows several of the 30 primer pairs found for human gene MDM1. Results can be downloaded in tab-separated format through the ‘Download’ link. The search is restricted to an organism, Ensemble release, and a maximum number of lines (the smaller the number, the faster the query). Each result line corresponds to a single primer pair, and displays its unique ID, the gene, and transcript(s) it targets, its star-based rank (among the best three pairs found for the gene), the fraction of isoforms it covers, the amplicon length, the primer sequences and their respective melting temperatures, and the Ensembl annotation for the gene (KNOWN or NOVEL). The last two columns provide respectively warnings if the primer search did not work with standard parameters and a link to a primer pair-specific page shown in Figure 3.