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. 2016 May 31;173(1):240–255. doi: 10.1104/pp.16.00095

Figure 6.

Figure 6.

Localization of DPW2. A to C, Fluorescence micrographs of the control anther at stage 8. Images taken from the GFP (green; A), chlorophyll autofluorescence (magenta; B), and merged channels (C). D to F, Fluorescence micrographs of stage 8 anther in transgenic lines expressing DPW2pro:DPW2CDS-GFP. Images were taken from the GFP (green; D), chlorophyll autofluorescence (magenta; E), and merged channels (F). Arrows in D and F indicate the microspores located in the locule of the anther. G to I, Magnification of the boxed region in D, E, and F, respectively. J to L, Magnification of the cells from the tapetum in G, H, and I, respectively. M to P, A rice protoplast expressing pA7-DPW2CDS-GFP, showing images from GFP fluorescence (green; M), chlorophyll autofluorescence (magenta; N), bright field (O), and the merged image (P), respectively. Q to S, A tobacco epidermal cell expressing 35Spro:DPW2CDS-GFP, showing images from GFP fluorescence (green; Q), bright field (R), and the merged image (S). Bars = 100 µm in A to F, 10 µm in G to I, 5 µm in J to P, and 20 µm in Q to S.