Analysis of anther wax and cutin in the wild type and the ipe1 mutant. A, Total cutin and wax amounts per unit of surface area (ng mm−2) in wild-type (black bars) and ipe1 (white bars) anthers. Error bars indicate sd (n = 5). B, Wax constituent amounts per unit of surface area (ng mm−2) in wild-type (black bars) and ipe1 (white bars) anthers. Error bars indicate sd (n = 5). C, Cutin constituent amounts per unit of surface area (ng mm−2) in wild-type (black bars) and ipe1 (white bars) anthers. Error bars indicate sd (n = 5). Compound names are abbreviated as follows: C16:1 acid, 7-hexadecenoic acid; C16:0 acid, hexadecanoic acid; C18:0 acid, octadecanoic acid; C18:1 acid, oleic acid; C18:2 acid, linoleic acid; C18:3 acid, linolenic acid; C20:0 acid, eicosanoic acid; C22:0 acid, docosanoic acid; C24:0 acid, tetracosanoic acid; C26:0 acid, hexacosanoic acid; C16:0 β-OH acid, 2-hydroxy-hexadecanoic acid; C18:0 β-OH acid, 2-hydroxy-octadecanoic acid; C22:0 β-OH acid, 2-hydroxy-docosanoic acid; C24:0 β-OH acid, 2-hydroxy-tetracosanoic acid; C26:0 β-OH acid, 2-hydroxy-hexacosanoic acid; C16:0 ω-OH acid, 16-hydroxy-hexadecanoic acid; C18:1 ω-OH acid, 18-hydroxy-oleic acid; C18:2 ω-OH acid, 18-hydroxy-linoleic acid; C18:0 9/10 di-OH acid, 9,10-dihydroxy-octadecanoic acid; C18:0 9/10 di-OH diacid, 9,10-dihydroxy-octadecanoic-1,18-dioic acid; C18:0 tri-OH acid, 9,10,18-trihydroxy-octadecanoic acid; C16:0 diacid, hexadecanoic-1,16-dioic acid; and C18:2 diacid, linoleic-1,18-dioic acid.