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. 2016 Nov 29;173(1):836–852. doi: 10.1104/pp.16.00949

Figure 9.

Figure 9.

SlRd2 dimerization is required for its interaction with SlCipk6. A, Y2H assay performed with SlCipk6 and SlCipk6(T1172D) in the bait vector pEG202 and SlRd2Δdim in the prey vector pJG4-5. Clones were transformed into EGY48 strain, grown in liquid culture, and spotted at OD600 = 0.1 and 0.01 on selective medium. Growth and blue patches indicates interaction. A Y2H experiment performed with SlRd2 and SlCipk6 was used as a control. B, Fluorescence images of N. benthamiana epidermal cells expressing SlRd2Δdim-YFPC and SlCipk6-YFPN. YFP signal was not reconstituted in the BiFC experiment (a), confirming that SlRd2 dimerization is required for the interaction with SlCipk6 in planta. A BiFC experiment performed with SlRd2 and SlCipk6 was used as a control (b). Bars = 10 μm.