Figure 2.

Defective pollen coats of mon1 result in decreased pollen adhesion to the stigma and a slower pollen hydration process. A to D, Fewer pollen grains were released and attached to the stigma in the mon1 mutant as shown by light and SEM imaging. Bars = 1 mm in A and B and 20 μm in C and D. E to H, Compared with the wild type (E), pollen (indicated by arrows) from the mon1 mutant (F) showed a slower hydration process when pollinated on the wild-type stigmatic papilla under low-humidity conditions (<40%). High humidity (>80%) partially rescued this defect of mon1 (G). Wild-type pollen showed a normal hydration process when pollinated on the mon1 stigmatic papilla under low-humidity conditions (H). Bars = 10 μm. WT, Wild type.