Figure 2.

Silencing of RhPR10.1 promotes flower senescence. A, Expression of the RhPR10 genes in RhPR10.1-silenced and TRV control flowers was analyzed by qRT-PCR. B, The time of flower opening (stages 1–4) and retention of ornamental value (stages 5 and 6) were recorded. C, Flower phenotypes were recorded and photographed every day. Stages of rose flowering were defined as follows: stage 1, bud with completely opened sepal; stage 2, bud with the outmost petal layer loosened; stage 3, flower with the outmost petal layer opened; stage 4, flower with the inner petal layer loosened; stage 5, fully opened flower; and stage 6, flower with fading color and loss of ornamental value. The results are the means of three biological replicates with standard deviations. Asterisks indicate statistically significant differences (Student’s t test, *P < 0.05, **P < 0.01).