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. 2016 Nov 22;173(1):853–862. doi: 10.1104/pp.16.01064

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Figure 6. — RhHB6 binds to a cis-element in the promoter of RhPR10.1. A, Transactivation activity of RhHB6 to the RhPR10.1 promoter in yeast. Wild-type and mutant probes were derived from the RhPR10.1 promoter. The wild-type cis-element and its nucleotide substitutions in the mutant versions are underlined. B, EMSA was used to analyze the interaction of GST-RhHB6 and a biotin-labeled probe. Purified protein (3 µg) samples were incubated with 25 pm of the biotin-labeled wild-type probe. Nonlabeled probes at 10- and 100-fold concentrations were added for the competition test. Images are representative of three independent experiments.