. 2016 Nov 8;173(1):390–416. doi: 10.1104/pp.16.01240

Table I. Acetyl-CoA and lipid metabolism proteins identified in membranes of nitrogen-depleted E. oleoabundans.

Acetyl-CoA and lipid metabolism proteins identified with at least two unique peptides in two or more biological replicates of total microsomal membrane samples are described. Very-low-abundance proteins that were identified exclusively in one biological replicate and/or in FFZE membrane fractions are highlighted with asterisks. The calculated molecular masses are shown, together with the corresponding protein homologs in the model organisms C. reinhardtii (Cr) and Arabidopsis (At) and their corresponding percentage identity values (%ID). Common abbreviations (Abbr.) of the identified proteins are provided. Subcellular localizations were predicted (Pr) using PredAlgo. Curated cell locations (Cu) were established according to the protein homologs. References are provided for protein homologs whose cell locations have been demonstrated experimentally (NA, not available). C, Chloroplast; Cy, cytoplasm; FAE, fatty acid elongase complex; FAS, fatty acid synthase complex; M, mitochondria; O, other; PM, plasma membrane; PX, peroxisome; SP, signal peptide.

Protein Identifier	Curated Description	Abbr.	Molecular Mass	EC No.	Best Cr Homolog		Best At Homolog		Subcellular Localization
Protein Identifier	Curated Description	Abbr.	Molecular Mass	EC No.	JGI Version 5.5 Identifier	%ID	The Arabidopsis Information Resource Version 10 Identifier	%ID	Pr	Cu	Reference
Acetyl-CoA synthesis
m.73526	Pyruvate dehydrogenase E1 component subunit α, chloroplastic*^a	PDH	47	1.2.4.1.	Cre02.g099850.t1.1	75	AT1G01090	70	C	C^b	Terashima et al. (2010)
m.73526		E1α	47	1.2.4.1.	Cre02.g099850.t1.1	75	AT1G01090	70	C	C^b	Terashima et al. (2010)
m.371437	Pyruvate dehydrogenase E1 component subunit β, chloroplastic	PDH	41	1.2.4.1.	Cre03.g194200.t1.2	86	AT1G30120	69	C	C^b	Terashima et al. (2010)
m.371437		E1β	41	1.2.4.1.	Cre03.g194200.t1.2	86	AT1G30120	69	C	C^b	Terashima et al. (2010)
m.130266	Pyruvate dehydrogenase E2 component, chloroplastic	PDH E2	50	2.3.1.12	Cre03.g158900.t1.2	74	AT3G25860	54	C	C^b	Terashima et al. (2010)
m.324850	Pyruvate dehydrogenase E3 component, chloroplastic	PDH E3	63	1.8.1.4.	Cre01.g016514.t1.1	72	AT3G16950	60	C	C^b	Terashima et al. (2010)
m.396299	ATP-citrate synthase α-chain protein*^c	ACLA	47	2.3.3.8.	Cre05.g241850.t1.2	62	AT1G10670	65	SP	Cy^b	Fatland et al. (2002)
m.391431	ATP-citrate synthase β-chain protein	ACLB	76	2.3.3.8.	Cre02.g088600.t1.2	77	AT5G49460	75	O	Cy^b	Fatland et al. (2002)
Lipid metabolism
Acyl-lipid biosynthesis
m.212887	Acetyl-CoA carboxylase carboxyl transferase subunit α, chloroplastic	ACC	60	6.4.1.2.	Cre12.g519100.t1.2	73	AT2G38040	54	C	C^b	Terashima et al. (2010)
m.212887		α-CT	60	6.4.1.2.	Cre12.g519100.t1.2	73	AT2G38040	54	C	C^b	Terashima et al. (2010)
gi\|3023244	Acetyl-CoA carboxylase carboxyl transferase subunit β, chloroplastic*^a	ACC	47	6.4.1.2.	Cre12.g484000.t1.1	75	ATCG00500	61	O	C^b	Terashima et al. (2010)
gi\|3023244		β-CT	47	6.4.1.2.	Cre12.g484000.t1.1	75	ATCG00500	61	O	C^b	Terashima et al. (2010)
m.390372	Biotin carboxylase, chloroplastic	ACC BCR	61	6.3.4.14 6.4.1.2.	Cre08.g359350.t1.2	80	AT5G35360	74	C	C^b	Terashima et al. (2010)
m.332618	3-Hydroxyacyl-[acyl-carrier-protein] dehydratase, chloroplastic*^a	FAS HAD	25	4.2.1.59.	Cre03.g208050.t1.2	70	AT5G10160	54	M	C^b	Terashima et al. (2010)
m.179214	Enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic*^c	FAS ENR	39	1.3.1.9.	Cre06.g294950.t1.1	82	AT2G05990	76	C	C^b	Terashima et al. (2010)
m.84123	β-Ketoacyl-CoA reductase*^c	FAE KCR	39	1.1.1.330.	Cre09.g392430.t1.1	32	AT1G67730	43	SP	E^b	Beaudoin et al. (2009)
m.368848	Long-chain acyl-CoA synthetase A	LCS	71	6.2.1.3	Cre13.g566650.t2.1	58	AT4G23850	48	O	LD^b	Moellering and Benning (2010)
m.224985	Long-chain acyl-CoA synthetase B	LCS	76	6.2.1.3	Cre13.g566650.t2.1	47	AT4G11030	43	SP	LD^b	Moellering and Benning (2010)
m.371326	Long-chain acyl-CoA synthetase C	LCS	67	6.2.1.3	Cre12.g507400.t1.2	63	AT5G27600	53	M	M^d	NA
Isoprenoid biosynthesis via the mevalonate pathway
m.149328	Acetyl-CoA acetyltransferase*^a	ACAT	51	2.3.1.9.	Cre02.g146050.t1.2	64	AT5G47720	59	M	Cy^b	Carrie et al. (2007)
Glycerolipid biosynthesis
m.241864	Glycerol-3-phosphate acyltransferase*^e	GPAT	54	2.3.1.15.	Cre06.g273250.t1.2	55	AT5G60620	60	O	E^b, LD^b	Gidda et al. (2009); Nguyen et al. (2011)
m.357823	1-Acyl-sn-glycerol-3-phosphate acyltransferase, chloroplastic*^a	LPAAT	37	2.3.1.51.	Cre09.g398289.t1.1	63	AT4G30580	56	C	C^b, LD^b	Ferro et al. (2010); Nguyen et al. (2011)
m.250190	Acyltransferase family protein*^c		54	NA	NA	NA	NA	NA	C	U	NA
Lipid signaling
m.34057	Phosphatidylinositol 4-kinase α*^a	PI4Kα	213	2.7.1.67.	Cre05.g245550.t1.1	45	AT1G49340	39	O	PM^b, LD^b	Nguyen et al. (2011); Zhang and Peck (2011)
m.226782	Sac1p-like phosphoinositide phosphatase*^a	SAC1	69	3.1.1.-	Cre09.g388750.t1.2	43	AT3G51460	39	O	E^b, LD^b	Despres et al. (2003); Nguyen et al. (2011)
LD structural proteins
m.413736	Major lipid droplet protein	MLDP	28	NA	Cre12.g491550.t1.2	24	NA	NA	O	LD^b	Davidi et al. (2012)
m.392627	Probable plastid-lipid associated protein A, chloroplastic	PLAP	59	NA	Cre07.g325736.t1.1	42	AT5G19940	32	M	C^b	Terashima et al. (2010)
m.50827	Probable plastid-lipid associated protein B, chloroplastic	PLAP	42	NA	Cre03.g189300.t1.1	44	AT4G04020	48	M	C^b	Ferro et al. (2010)
m.244306	Probable plastid-lipid associated protein C, chloroplastic	PLAP	23	NA	Cre03.g188650.t1.2	62	AT3G26070	58	C	C^b	Terashima et al. (2010)
gi\|132270	Rubber elongation factor protein*^a	REF	15	NA	NA	NA	AT3G05500	48	O	LD^b	Horn et al. (2013)
Lipid trafficking
m.216464	Membrane-associated 30-kD protein, chloroplastic	VIPP1	33	NA	Cre13.g583550.t1.2	54	AT1G65260	54	C	C^b	Nordhues et al. (2012)
m.362261	Protein trigalactosyldiacylglycerol 2, chloroplastic	TGD2	44	NA	Cre16.g694400.t1.2	57	AT3G20320	40	O	C^b	Terashima et al. (2010)
m.116135	Phospholipid-transporting ATPase*^a	ALA	159	3.6.3.1.	Cre16.g656500.t1.1	32	AT1G59820	33	O	E^b, PM^b	Poulsen et al. (2008); Mitra et al. (2009)
m.417181	ABC transporter G family member A	ABCG	70	3.6.3.-	Cre07.g313250.t1.2	57	AT3G55100	31	O	PM^d	NA
m.306564	ABC transporter G family member C	ABCG	69	3.6.3.-	Cre07.g313250.t1.2	55	AT3G55100	29	O	PM^d	NA
m.117336	Chloroplast J-like domain-containing protein	CJD1	30	NA	Cre03.g171100.t1.1	34	AT1G08640	26	C	C^b	Ajjawi et al. (2011)
Lipases and fatty acid β-oxidation
m.419400	Putative triacylglycerol lipase*^a	TGL	47	3.1.1.3.	Cre07.g348550.t1.1	42	AT5G67050	37.3	SP	Cy^d	NA
m.225854	Acyl-CoA oxidase A, peroxisomal*^a	ACX	75	1.3.3.6.	Cre05.g232002.t1.1	66	AT5G65110	57.9	O	PX^b	Stabenau et al. (1984)
m.366023	Acyl-CoA oxidase B, peroxisomal*^e	ACX	74	1.3.3.6.	Cre11.g467350.t1.2	62	AT1G06290	36	O	PX^b	Stabenau et al. (1984)
m.420224	Fatty acid β-oxidation multifunctional protein, peroxisomal*^c	MFP	77	4.2.1.17. 1.1.1.35.	Cre16.g695050.t1.2	66	AT3G06860	55	SP	PX^b	Stabenau et al. (1984)
m.356362	2,4-Dienoyl-CoA reductase, peroxisomal*^c	RED	35	1.3.1.4.	Cre17.g731850.t1.2	64	AT3G12800	53	SP	PX^b	Reumann et al. (2009)

Protein identified exclusively in FFZE membrane fractions ^bExperimental evidence of subcellular location is available. ^cProtein identified in one biological replicate of total microsomal samples and in FFZE membrane fractions. ^dExperimental evidence of subcellular location is not available. ^eProtein identified in only one biological replicate of total microsomal samples.