Figure 3.

Comparisons of low-β with CEZ. A, Complementation test of low-β and CEZ with a panel of Cmor-Arg homozygous lines: NY, NA, ED, TAD, and PDS. The CmOr-His homozygous lines INB, DUL, and CEZ served as controls. Images of the female parent fruits are in the top row. The male parent was either low-β or CEZ (fruits shown at right). F1 hybrid fruits of the crosses with low-β (a–h) and with CEZ (i–p) are shown. In a to e, nonorange fruits in hybrids indicated that low-β is allelic to CmOr. B, Aligned partial protein sequences of CmOR-His (CEZ) and CmOR-Arg (TAD) and the low-β OR protein with its premature stop codon (asterisk) at position 163. The golden SNP (His to Arg) at position 108 is highlighted. C, CmOr mRNA digital expression in CEZ and low-β developing fruit mesocarp. Columns represent average reads per kilobase of exon model per million mapped reads (RPKM) ± se of three independent biological repeats. D, CmOR protein levels in CEZ and low-β mutant at 20 and 30 DAA and in mature melon fruit. CmOR protein was detected by immunoblot analysis. A Ponceau S-stained membrane is shown below. E, Yeast-split ubiquitin analysis (-LW = selective medium lacking Leu and Trp; -LWAH+Met = selective medium lacking Leu, Trp, adenine, and His and supplemented with 150 μm Met).