Figure 6.

PSY protein and phytoene levels in melon lines. A, Western blot showing PSY protein levels at 30 DAA in six melon inbred lines and low-β with actin signals used as a loading control; the fruit flesh phenotypes at 30 DAA are shown below. Each sample is a pool of six fruits from six different plants. B, PSY protein levels at 5, 10, 20, and 30 DAA of green flesh and orange flesh bulked segregants originating from a cross between DUL and TAD and sharing the same fruit flesh color. Each sample is a bulk of 75 fruits originating from 25 different F3 families. C, Phytoene accumulated in 30-DAA fruit discs upon NF treatment. Phytoene is expressed in µg g⁻¹ fresh weight (FW) of treated fruit flesh at 24 h of treatment. Values are means ± sd of three biological repeats. Although PSY protein and phytoene levels upon NF treatment are quite similar, β-carotene content in CmOR-His is much higher than in Cmor-Arg melons.