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. 2016 Nov 16;173(1):771–787. doi: 10.1104/pp.16.01404

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Figure 7. — CRK28 self-associates in N. benthamiana and also associates with CRK29. A, Immunoblot showing the Co-IP of CRK28-HA with CRK28-FLAG in the presence or absence of flg22. 35S:CRK28-HA and 35S:CRK28-FLAG were coinfiltrated into N. benthamiana leaves, and 20 hpi, the leaves were infiltrated with either flg22 or water and leaf samples were collected 5 min later for IP. The asterisk shows an anti-FLAG cross-reacting band. B, Co-IP of CRK28-HA along with CRK29-FLAG in N. benthamiana. The experiments were performed as described in A. An anti-FLAG cross-reacting band is present in the first and fifth lanes. These experiments were repeated three times with similar results.