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. 2016 Nov 23;173(1):715–727. doi: 10.1104/pp.16.01504

Figure 6.

Figure 6.

Presence of the TATA-box correlates with an increase in transcriptional activation by specific TFIID protein binding. A, Yeast one-hybrid assay showing the activity of LacZ reporters driven by the FUE and FUI promoters and activated by the activation domain (AD) fused with MDP0000495467 or MDP0000939369. Empty vectors pJG4-5 and pLacZi were used as negative controls. B, Gel-shift analysis of MDP0000939369 binding to the promoters of FUE. EMSAs of GST-labeled probe fused with TFIID protein (MDP0000939369); DNA probes represent the FUE or FUI (probe sequences are shown in Supplemental Table S2). Protein/DNA complexes are indicated by arrows. C, Relative expression of MDP0000939369 in M. xiaojinensis and M. baccata supplied with 0 μm Fe2+ or 40 μm Fe2+ for 0, 3, and 6 d. D, Correlation analysis between relative expression levels of IRT1 and MDP0000939369. E, Schematic model of the regulation of IRT1 expression by the TATA-box insertion regulatory module.