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. 2016 Nov 17;173(1):600–613. doi: 10.1104/pp.16.01539

Figure 4.

Figure 4.

Subcellular localization of YFP-HR1, YFP-HR2, YFP-HR3, and YFP-HR4. Except for YFP-HR4, which was transiently expressed in leaves of N. benthamiana, the rest were stably expressed in Arabidopsis Col-0 (At Col-0). A, Representative images showing cytoplasmic and likely plasma membrane localization of indicated fusion proteins expressed from the 35S promoter in leaves of Arabidopsis transgenic plants (for HR1, HR2 and HR3) or agroinfiltrated N. benthamiana plants (for HR4). B, A representative image showing colocalization of YFP-HR3 with AtPIP2A-mCherry in N. benthamiana epidermal cells when transiently coexpressed. C, Representative images showing likely EHM-localization of indicated fusion proteins expressed from the 35S promoter at 2 dpi with Gc UCSC1. D, EHM-localization of YFP-HR3 expressed from the HR3 NP in At Col-0 at 2 dpi with Gc UCSC1. Bar, 20 μm in A; 50 μm in B; 10 μm in C and D. H, Haustorium; Nb, N. benthamiana.