Figure 5.
YFP-HR3 is localized to the plasma membrane, the EHM, and the papilla. A, Representative image showing that YFP-HR3 is localized to the PM (arrow), the EHM (labeled by RPW8.2-RFP), and the papilla (arrowhead). Inset is a close-up view of the transverse section of a fungal penetration site where both YFP-HR3 and callose focally accumulate. Note the heterogeneous distribution of YFP-HR3. B, Representative isolated haustorial complex with an intact EHM labeled by both YFP-HR3 and RPW8.2-RFP. C, PM-localization of GFP-SYP131 in the epidermal cells containing haustoria (arrows). D, Maximum projection of a Z-stack image Z-stack image showing YFP-RPW8.2 is localized to the EHM but not in the papilla where callose is deposited (arrow). Bar, 10 μm in A, B, and D; 50 μm in C. Cp, Chloroplast.