Figure 6.

Brassica RPW8 homologs are also localized to the EHM and the papilla. A and B, Representative powdery mildew-infected T1 plants expressing BoHRa-YFP (A) or BoHRb-YFP (B) from the RPW8.2 promoter (upper panel) and total number of T1 transgenic plants from each indicated category (lower panel). Arrows indicate plants with spontaneous cell death and resistance to powdery mildew (disease reaction score, DR = 0 to 1; Xiao et al., 2005); arrowheads indicate plants displaying enhanced mildew resistance without spontaneous cell death (DR = 1 to 2); asterisks indicate plants susceptible to powdery mildew (DR = 2 to 3 or 3). Pictures were taken at 9 dpi. C and D, Representative images showing accumulation of BoHRa-YFP (C) or YFP-BoHRa (D) in the papilla. E, Representative image showing EHM-localization of YFP-BoHRa. Bar, 10 μm in C to E.