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. 2016 Nov 17;173(1):600–613. doi: 10.1104/pp.16.01539

Figure 7.

Figure 7.

The EHM is physically separable from the PM and the papilla. Gc UCSC-infected leaves expressing YFP-HR3 (from 35S) and RPW8.2-RFP (from the RPW8.2 promoter) were subjected to plasmolysis (0.5 M NaCl). A, Representative confocal image taken at 10 min after treatment. Note that the cytoplasm with YFP-HR3-labeled PM was shrunk around the seemingly unaffected haustorium labeled by both YFP-HR3 and RPW8.2-RFP. B and C, Representative confocal images taken at 30 min after treatment showing that the cytoplasm with YFP-HR3-labeled PM was nearly (B) or completely (C) detached from the haustorial complex with an intact EHM labeled by YFP-HR3 and RPW8.2-RFP. Note that YFP-HR3 localized to the papilla stained by PI (arrows in C) was also unaffected. Bar, 10 μm in A to C. C, Cytoplasm; H, haustorium.